The book imparts insight into the principles and concepts of the high kinetic and structural resolution of fast chemical, biochemical and biophysical reactions of proteins with emphasis on protein folding. The study of fast protein folding reactions and the insight into the mechanisms that make folding such a highly efficient process have become extremely advanced since the recent development of new methods which enable not only kinetic resolution in the millisecond, microsecond and picosecond time range, but also significantly higher structural resolution. The pathways and structures of early folding events and the transition state structures of fast folding proteins can now be studied in far more detail. Important techniques include site-directed mutagenesis, F-value analysis, NMR, circular dichroism, fluorescence, LASER spectroscopy, temperature-jumping, ultrafast mixing, stopped flow, dielectric relaxation, acoustic relaxation and pressure jump techniques.
First methods book which includes many detailed descriptions
Absolutely needed and thus timely for the scientific community
Comprises 15% more content and includes the mentioned special features
First methods book which includes many detailed descriptions
Absolutely needed and thus timely for the scientific community
Comprises 15% more content and includes the mentioned special features