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High Quality Content by WIKIPEDIA articles! Protein methods are the techniques used to study proteins. There are genetic methods for studying proteins, methods for detecting proteins, methods for isolating and purifying proteins and other methods for characterizing the structure and function of proteins, often requiring that the protein first be purified. Conceptual translation- many proteins are never directly sequenced, but their sequence of amino acids is known by "conceptual translation" of a known mRNA sequence. See Genetic code. Site-directed mutagenesis allows new variants of proteins to be produced and tested for how structural changes alter protein function. Insertion of protein tags such as the His-tag. See also: Green fluorescent protein.