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High Quality Content by WIKIPEDIA articles! Protein?protein interaction prediction is a field combining bioinformatics and structural biology in an attempt to identify and catalog physical interactions between pairs or groups of proteins. Understanding protein?protein interactions is important for the investigation of intracellular signaling pathways, modelling of protein complex structures and for gaining insights into various biochemical processes. Experimentally, physical interactions between pairs of proteins can be inferred from a variety of experimental techniques, including yeast two-hybrid systems, protein-fragment complementation assays (PCA), affinity purification/mass spectrometry, protein microarrays and fluorescence resonance energy transfer (FRET). Efforts to experimentally determine the interactome of numerous species are ongoing, and a number of computational methods for interaction prediction have been developed in recent years.