Protein-Protein Interactions
Methods and Protocols
Herausgeber: Mukhtar, Shahid
Protein-Protein Interactions
Methods and Protocols
Herausgeber: Mukhtar, Shahid
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This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based approaches as well as gene expression and machine learning methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists…mehr
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This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based approaches as well as gene expression and machine learning methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein-Protein Interactions: Methods and Protocols serves as an ideal guide for researchers working in protein science and beyond.
Produktdetails
- Produktdetails
- Methods in Molecular Biology Nr.2690
- Verlag: Springer US
- 2023
- Seitenzahl: 504
- Erscheinungstermin: 15. Juli 2024
- Englisch
- Abmessung: 254mm x 178mm x 28mm
- Gewicht: 938g
- ISBN-13: 9781071633298
- ISBN-10: 1071633295
- Artikelnr.: 71261716
- Methods in Molecular Biology Nr.2690
- Verlag: Springer US
- 2023
- Seitenzahl: 504
- Erscheinungstermin: 15. Juli 2024
- Englisch
- Abmessung: 254mm x 178mm x 28mm
- Gewicht: 938g
- ISBN-13: 9781071633298
- ISBN-10: 1071633295
- Artikelnr.: 71261716
Yeast
Two
Hybrid Technique to Identify Protein
Protein Interactions.
Cytotrap: An Innovative Approach for Protein
Protein Interaction Studies for Cytoplasmic Proteins.
Analyzing Protein
Protein Interactions Using the Split
Ubiquitin System.
Detection of Protein
Protein Interactions Utilizing the Split
Ubiquitin Membrane
Based Yeast Two
Hybrid System.
Preparation and Utilization of a Versatile GFP
Protein Trap
Like System for Protein Complex Immunoprecipitation in Plants.
Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG
and HA
Tagged Bait Proteins.
Affinity Purification
Mass Spectroscopy (AP
MS) and Co
Immunoprecipitation (Co
IP) Technique to Study Protein
Protein Interactions.
Co
Immunoprecipitation
Based Identification of Effector
Host Protein Interactions from Pathogen
Infected Plant Tissue.
Co
Immunoprecipitation for Assessing Protein
Protein Interactions in Agrobacterium
Mediated Transient Expression System in Nicotiana benthamiana .
Detection of Protein
Protein Interactions Using Glutathione
S
Transferase (GST) Pull
Down Assay Technique.
Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein
Protein Interactions in Living Cells.
Detecting Protein
Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) and Luciferase Complementation Assays (LCA).
Forster Resonance Energy Transfer (FRET) to Visualize Protein
Protein Interactions in the Plant Cell.
Dynamic Proximity Tagging in Living Plant Cells with Pupylation
Based Interaction Tagging.
Characterization of Small Molecule
Protein Interactions Using SPR Method.
High
Throughput Protein
Protein Interactions Screening Using Pool
Based Liquid Yeast Two
Hybrid Pipeline.
Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two
Hybrid (Y2H) Protocol to Evaluate Networks.
An Interactome Assay for Detecting Interactions between Extracellular Domains of Receptor Kinases.
Next
Generation YeastTwo
Hybrid Screening to Discover Protein
Protein Interactions.
Bioinformatic Analysis of Yeast Two
Hybrid Next
Generation Interaction Screen Data.
Discovering Protein
Protein Interactions Using Co
Fractionation
Mass Spectrometry with Label
Free Quantitation.
Protein
Protein Interaction Network Mapping by Affinity Purification Cross
Linking (AP
CXL
MS) Proteomics.
Protein Interaction Screen on a Peptide Matrix (PrISMa).
Analyzing Protein Interactions by MAC
Tag Approaches.
Identification and Quantification of Affinity
Purified Proteins with MaxQuant, Followed by the Discrimination of Nonspecific Interactions with the CRAPome Interface.
Cataloguing Protein Complexes In Planta Using TurboID
Catalyzed Proximity Labeling.
A Data
Driven Signaling Network Inference Approach for Phosphoproteomics.
Pairwise and Multi
Chain Protein Docking Enhanced Using LZerD Web Server.
Predicting Protein Interaction Sites Using PITHIA.
Using PlaPPISite to Predict and Analyze Plant Protein
ProteinInteraction Sites.
Machine Learning Methods for Virus
Host Protein
Protein Interaction Prediction.
Protein
Protein Interaction Network Exploration Using Cytoscape.
Search, Retrieve, Visualize, and Analyze Protein
Protein Interactions from Multiple Databases: A Guide for Experimental Biologists.
Centrality Analysis of Protein
Protein Interaction Networks Using R.
Protein
Protein Interaction Network Analysis Using NetworkX.
Building Protein
Protein Interaction Graph Database Using Neo4j.
Two
Hybrid Technique to Identify Protein
Protein Interactions.
Cytotrap: An Innovative Approach for Protein
Protein Interaction Studies for Cytoplasmic Proteins.
Analyzing Protein
Protein Interactions Using the Split
Ubiquitin System.
Detection of Protein
Protein Interactions Utilizing the Split
Ubiquitin Membrane
Based Yeast Two
Hybrid System.
Preparation and Utilization of a Versatile GFP
Protein Trap
Like System for Protein Complex Immunoprecipitation in Plants.
Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG
and HA
Tagged Bait Proteins.
Affinity Purification
Mass Spectroscopy (AP
MS) and Co
Immunoprecipitation (Co
IP) Technique to Study Protein
Protein Interactions.
Co
Immunoprecipitation
Based Identification of Effector
Host Protein Interactions from Pathogen
Infected Plant Tissue.
Co
Immunoprecipitation for Assessing Protein
Protein Interactions in Agrobacterium
Mediated Transient Expression System in Nicotiana benthamiana .
Detection of Protein
Protein Interactions Using Glutathione
S
Transferase (GST) Pull
Down Assay Technique.
Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein
Protein Interactions in Living Cells.
Detecting Protein
Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) and Luciferase Complementation Assays (LCA).
Forster Resonance Energy Transfer (FRET) to Visualize Protein
Protein Interactions in the Plant Cell.
Dynamic Proximity Tagging in Living Plant Cells with Pupylation
Based Interaction Tagging.
Characterization of Small Molecule
Protein Interactions Using SPR Method.
High
Throughput Protein
Protein Interactions Screening Using Pool
Based Liquid Yeast Two
Hybrid Pipeline.
Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two
Hybrid (Y2H) Protocol to Evaluate Networks.
An Interactome Assay for Detecting Interactions between Extracellular Domains of Receptor Kinases.
Next
Generation YeastTwo
Hybrid Screening to Discover Protein
Protein Interactions.
Bioinformatic Analysis of Yeast Two
Hybrid Next
Generation Interaction Screen Data.
Discovering Protein
Protein Interactions Using Co
Fractionation
Mass Spectrometry with Label
Free Quantitation.
Protein
Protein Interaction Network Mapping by Affinity Purification Cross
Linking (AP
CXL
MS) Proteomics.
Protein Interaction Screen on a Peptide Matrix (PrISMa).
Analyzing Protein Interactions by MAC
Tag Approaches.
Identification and Quantification of Affinity
Purified Proteins with MaxQuant, Followed by the Discrimination of Nonspecific Interactions with the CRAPome Interface.
Cataloguing Protein Complexes In Planta Using TurboID
Catalyzed Proximity Labeling.
A Data
Driven Signaling Network Inference Approach for Phosphoproteomics.
Pairwise and Multi
Chain Protein Docking Enhanced Using LZerD Web Server.
Predicting Protein Interaction Sites Using PITHIA.
Using PlaPPISite to Predict and Analyze Plant Protein
ProteinInteraction Sites.
Machine Learning Methods for Virus
Host Protein
Protein Interaction Prediction.
Protein
Protein Interaction Network Exploration Using Cytoscape.
Search, Retrieve, Visualize, and Analyze Protein
Protein Interactions from Multiple Databases: A Guide for Experimental Biologists.
Centrality Analysis of Protein
Protein Interaction Networks Using R.
Protein
Protein Interaction Network Analysis Using NetworkX.
Building Protein
Protein Interaction Graph Database Using Neo4j.
Yeast
Two
Hybrid Technique to Identify Protein
Protein Interactions.
Cytotrap: An Innovative Approach for Protein
Protein Interaction Studies for Cytoplasmic Proteins.
Analyzing Protein
Protein Interactions Using the Split
Ubiquitin System.
Detection of Protein
Protein Interactions Utilizing the Split
Ubiquitin Membrane
Based Yeast Two
Hybrid System.
Preparation and Utilization of a Versatile GFP
Protein Trap
Like System for Protein Complex Immunoprecipitation in Plants.
Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG
and HA
Tagged Bait Proteins.
Affinity Purification
Mass Spectroscopy (AP
MS) and Co
Immunoprecipitation (Co
IP) Technique to Study Protein
Protein Interactions.
Co
Immunoprecipitation
Based Identification of Effector
Host Protein Interactions from Pathogen
Infected Plant Tissue.
Co
Immunoprecipitation for Assessing Protein
Protein Interactions in Agrobacterium
Mediated Transient Expression System in Nicotiana benthamiana .
Detection of Protein
Protein Interactions Using Glutathione
S
Transferase (GST) Pull
Down Assay Technique.
Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein
Protein Interactions in Living Cells.
Detecting Protein
Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) and Luciferase Complementation Assays (LCA).
Forster Resonance Energy Transfer (FRET) to Visualize Protein
Protein Interactions in the Plant Cell.
Dynamic Proximity Tagging in Living Plant Cells with Pupylation
Based Interaction Tagging.
Characterization of Small Molecule
Protein Interactions Using SPR Method.
High
Throughput Protein
Protein Interactions Screening Using Pool
Based Liquid Yeast Two
Hybrid Pipeline.
Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two
Hybrid (Y2H) Protocol to Evaluate Networks.
An Interactome Assay for Detecting Interactions between Extracellular Domains of Receptor Kinases.
Next
Generation YeastTwo
Hybrid Screening to Discover Protein
Protein Interactions.
Bioinformatic Analysis of Yeast Two
Hybrid Next
Generation Interaction Screen Data.
Discovering Protein
Protein Interactions Using Co
Fractionation
Mass Spectrometry with Label
Free Quantitation.
Protein
Protein Interaction Network Mapping by Affinity Purification Cross
Linking (AP
CXL
MS) Proteomics.
Protein Interaction Screen on a Peptide Matrix (PrISMa).
Analyzing Protein Interactions by MAC
Tag Approaches.
Identification and Quantification of Affinity
Purified Proteins with MaxQuant, Followed by the Discrimination of Nonspecific Interactions with the CRAPome Interface.
Cataloguing Protein Complexes In Planta Using TurboID
Catalyzed Proximity Labeling.
A Data
Driven Signaling Network Inference Approach for Phosphoproteomics.
Pairwise and Multi
Chain Protein Docking Enhanced Using LZerD Web Server.
Predicting Protein Interaction Sites Using PITHIA.
Using PlaPPISite to Predict and Analyze Plant Protein
ProteinInteraction Sites.
Machine Learning Methods for Virus
Host Protein
Protein Interaction Prediction.
Protein
Protein Interaction Network Exploration Using Cytoscape.
Search, Retrieve, Visualize, and Analyze Protein
Protein Interactions from Multiple Databases: A Guide for Experimental Biologists.
Centrality Analysis of Protein
Protein Interaction Networks Using R.
Protein
Protein Interaction Network Analysis Using NetworkX.
Building Protein
Protein Interaction Graph Database Using Neo4j.
Two
Hybrid Technique to Identify Protein
Protein Interactions.
Cytotrap: An Innovative Approach for Protein
Protein Interaction Studies for Cytoplasmic Proteins.
Analyzing Protein
Protein Interactions Using the Split
Ubiquitin System.
Detection of Protein
Protein Interactions Utilizing the Split
Ubiquitin Membrane
Based Yeast Two
Hybrid System.
Preparation and Utilization of a Versatile GFP
Protein Trap
Like System for Protein Complex Immunoprecipitation in Plants.
Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG
and HA
Tagged Bait Proteins.
Affinity Purification
Mass Spectroscopy (AP
MS) and Co
Immunoprecipitation (Co
IP) Technique to Study Protein
Protein Interactions.
Co
Immunoprecipitation
Based Identification of Effector
Host Protein Interactions from Pathogen
Infected Plant Tissue.
Co
Immunoprecipitation for Assessing Protein
Protein Interactions in Agrobacterium
Mediated Transient Expression System in Nicotiana benthamiana .
Detection of Protein
Protein Interactions Using Glutathione
S
Transferase (GST) Pull
Down Assay Technique.
Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein
Protein Interactions in Living Cells.
Detecting Protein
Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) and Luciferase Complementation Assays (LCA).
Forster Resonance Energy Transfer (FRET) to Visualize Protein
Protein Interactions in the Plant Cell.
Dynamic Proximity Tagging in Living Plant Cells with Pupylation
Based Interaction Tagging.
Characterization of Small Molecule
Protein Interactions Using SPR Method.
High
Throughput Protein
Protein Interactions Screening Using Pool
Based Liquid Yeast Two
Hybrid Pipeline.
Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two
Hybrid (Y2H) Protocol to Evaluate Networks.
An Interactome Assay for Detecting Interactions between Extracellular Domains of Receptor Kinases.
Next
Generation YeastTwo
Hybrid Screening to Discover Protein
Protein Interactions.
Bioinformatic Analysis of Yeast Two
Hybrid Next
Generation Interaction Screen Data.
Discovering Protein
Protein Interactions Using Co
Fractionation
Mass Spectrometry with Label
Free Quantitation.
Protein
Protein Interaction Network Mapping by Affinity Purification Cross
Linking (AP
CXL
MS) Proteomics.
Protein Interaction Screen on a Peptide Matrix (PrISMa).
Analyzing Protein Interactions by MAC
Tag Approaches.
Identification and Quantification of Affinity
Purified Proteins with MaxQuant, Followed by the Discrimination of Nonspecific Interactions with the CRAPome Interface.
Cataloguing Protein Complexes In Planta Using TurboID
Catalyzed Proximity Labeling.
A Data
Driven Signaling Network Inference Approach for Phosphoproteomics.
Pairwise and Multi
Chain Protein Docking Enhanced Using LZerD Web Server.
Predicting Protein Interaction Sites Using PITHIA.
Using PlaPPISite to Predict and Analyze Plant Protein
ProteinInteraction Sites.
Machine Learning Methods for Virus
Host Protein
Protein Interaction Prediction.
Protein
Protein Interaction Network Exploration Using Cytoscape.
Search, Retrieve, Visualize, and Analyze Protein
Protein Interactions from Multiple Databases: A Guide for Experimental Biologists.
Centrality Analysis of Protein
Protein Interaction Networks Using R.
Protein
Protein Interaction Network Analysis Using NetworkX.
Building Protein
Protein Interaction Graph Database Using Neo4j.