Protein Purification - Janson, Jan-Christer
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This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
- Gel filtration and ion exchange chromatography
- Affinity and covalent chromatography
- Reversed phase and hydrophobic interaction chromatography
- Ligands from combinatorial…mehr

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Produktbeschreibung
This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:

- Gel filtration and ion exchange chromatography

- Affinity and covalent chromatography

- Reversed phase and hydrophobic interaction chromatography

- Ligands from combinatorial libraries

- Electrophoresis in gels

- Immunoelectrophoresis and capillary electrophoresis

- Two-dimensional electrophoresis in proteomics

- Protein elution and blotting techniques

- Large-scale electrophoresis processes
The authoritative guide on protein purification-now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

High-resolution reversed-phase liquid chromatography

Electrophoresis in gels

Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

Affinity ligands from chemical and biological combinatorial libraries

Membrane separations

Refolding of inclusion body proteins from E. coli

Purification of PEGylated proteins

High throughput screening techniques in protein purification

The history of protein chromatography
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Autorenporträt
JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.