Jan-Christer Janson

Protein Purification

Principles, High Resolution Methods, and Applications
Ed. by Jan-Christer Janson

• Gebundenes Buch

Produktbeschreibung

This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:

- Gel filtration and ion exchange chromatography

- Affinity and covalent chromatography

- Reversed phase and hydrophobic interaction chromatography

- Ligands from combinatorial libraries

- Electrophoresis in gels

- Immunoelectrophoresis and capillary electrophoresis

- Two-dimensional electrophoresis in proteomics

- Protein elution and blotting techniques

- Large-scale electrophoresis processes

---

The authoritative guide on protein purification-now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

High-resolution reversed-phase liquid chromatography

Electrophoresis in gels

Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

Affinity ligands from chemical and biological combinatorial libraries

Membrane separations

Refolding of inclusion body proteins from E. coli

Purification of PEGylated proteins

High throughput screening techniques in protein purification

The history of protein chromatography

Produktdetails

• Wiley Series in Methods of Biochemical Analysis Vol.54
• Verlag: Wiley & Sons
• 3. Aufl.
• Seitenzahl: 544
• Erscheinungstermin: 22. März 2011
• Englisch
• Abmessung: 286mm x 221mm x 33mm
• Gewicht: 1495g
• ISBN-13: 9780471746614
• ISBN-10: 0471746614
• Artikelnr.: 22580132

Autorenporträt

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

Inhaltsangabe

Preface. Contributors. Part I. Introduction. 1.Introduction to Protein
Purification (Bo Ersson, Lars Rydén and Jan-Christer Janson). Part II.
Chromatography. 2. Introduction to Chromatography (Jan-Christer Janson and
Jan-Åke Jönsson). 3. Gel Filtration (Lars Hagel). 4. Ion Exchange
Chromatography (Evert Karlsson and Irwin Hirsh). 5. High-Resolution
Reversed-Phase Chromatography (Sylvia Winkel Pettersson). 6. Hydrophobic
Interaction Chromatography (Kjell-Ove Eriksson and Makonnen Belew). 7.
Immobilized Metal Ion Affinity Chromatography (Lennart Kågedal). 8.
Covalent Chromatpgraphy (Francisco Batista-Viera, Lars Rydén and Jan
Carlsson). 9. Affinity Chromatography (Francisco Batista-Viera,
Jan-Christer Janson and Jan Carlsson). 10. Affinity Ligands from Chemical
Combinatorial Libraries (Enrique Carredano and Herbert Baumann). 11.
Affinity Ligands from Biological Combinatorial Libraries (Per-Åke Nygren).
Part III. Other Separation Methods and Related Techniques. 12. Membrane
Separations (Joachim K. Walter, Zuwei Jin, Maik W. Jornitz and Uwe
Gottschalk). 13. Refolding of Inclusion Body Proteins from E. coli (Zhiguo
Su, Siannan Lu and Zheng Liu). 14. Purification of PEGylated Proteins
(Conan J. Fee and James M. Van Alstine). Part IV. Electrophoresis. 15.
Electrophoresis in Gels (Reiner Westermeier). 16. Conventional Isoelectric
Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients (Pier
Giorgio Righetti, Alessia Farinazzo, Elisa Fasoli and Sabina Carla
Righetti). 17. Two-Dimensional Electrophoresis in Proteomics (Reiner
Westermeier and Angelika Görg). 18. Protein Elution and Blotting Techniques
(Reiner Westermeier). 19. Capillary Electrophoretic Separations (Wolfgang
Thormann). Part V. Separation Method Optimization. 20. High Throughput
Screening Techniques on Protein Purification (Karol M. Lacki and Eggert
Brekkan). Index.