Beschreibung
Produktdetails
Einband
Taschenbuch
Erscheinungsdatum
15.08.2012
Verlag
LAP LAMBERT Academic PublishingSeitenzahl
224
Maße (L/B/H)
22/15/1,3 cm
Gewicht
350 g
Sprache
Englisch
ISBN
978-3-659-17202-1
Proteomics is a powerful technique for the large-scale identification of proteins. A number of proteomics approaches have been developed to study protein localization at steady state. Here we report a novel dynamic proteomics strategy to identify plasma membrane proteins that undergo retrograde transport to the trans-Golgi network. This strategy is based on the covalent modification of the plasma membrane proteome with a benzylguanine derivative. Only benzylguanine-tagged plasma membrane proteins that are transported via the retrograde route can covalently couple to a TGN-localized SNAP-tag fusion protein. The approach was validated step-by-step using a well explored retrograde cargo protein, the B-subunit of Shiga toxin. It was then extended in an unbiased manner to the proteomics format. This allowed identifying an endogenous protein that was one of the first retrograde cargo proteins to be described in the past. In conclusion, we have pioneered a dynamic proteomics approach that complements traditional approaches in the study of protein trafficking. Moreover, the concept that is described here is of generic nature and may be extended to other endocytic pathways.
Kundinnen und Kunden meinen
Verfassen Sie die erste Bewertung zu diesem Artikel
Helfen Sie anderen Kund*innen durch Ihre Meinung
Kurze Frage zu unserer Seite
Vielen Dank für dein Feedback
Wir nutzen dein Feedback, um unsere Produktseiten zu verbessern. Bitte habe Verständnis, dass wir dir keine Rückmeldung geben können. Falls du Kontakt mit uns aufnehmen möchtest, kannst du dich aber gerne an unseren Kund*innenservice wenden.
zum Kundenservice