Alan Longstaff, Patricia Revest

Protocols in Molecular Neurobiology

• Broschiertes Buch

Produktbeschreibung

Neurobiologists are bound to differ in their perceptions of what the discipline of molecular neurobiology should encompass. We have taken the view that molecular neurobiology should cover any aspect of brain science that uses the techniques of modern molecular biology, though we accept the fact that classification of a technique as a "biochemical or "molecular biological one is in itself somewhat arbitrary. Each chapter of this volume sets out to identify a clear problem in neurobiology and to place it in its context within the literatu- i.e., indicating how the solution of the problem will advance knowledge in the field. The core of the chapter then details the approaches taken to solve the problem, in sufficient detail that the reader can appre- ate exactly why a specific strategy was adopted and how it was executed. Each chapter also includes detailed protocols providing all the inf- mation necessary to reproduce the technique and its results in any appropriately equipped laboratory.

Produktdetails

• Methods in Molecular Biology Vol.13
• Verlag: Springer, Berlin
• 1993
• Seitenzahl: 373
• Englisch
• Abmessung: 230mm x 163mm x 30mm
• Gewicht: 1g
• ISBN-13: 9780896031999
• ISBN-10: 0896031993
• Artikelnr.: 24879944

Inhaltsangabe

Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis. The Isolation of Genomic DNA from Invertebrates. Analysis of Mitochondrial DNA Mutations. The Design and Use of Oligonucleotides. The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences. Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization. Generation of Isoform-Specific Antisera from Cloned cDNAs: Application to Multiple Forms of the Na/K-ATPase Expressed in Rat Brain. Determination of Transcriptional Initiation Sites and Their Usage in the Nervous System. Mapping of Trans-Acting Factor Binding in the Nervous System. In Situ Hybridization to Brain Tissue Sections Using Labeled Single-Strand Complementary RNA Probes. In Situ Hybridization Histochemistry Using Alkaline Phosphatase-Labeled Oligodeoxynucleotide Probe. The Identification of Neuropeptide Gene Regulatory Elements in Transgenic Mice. Expression of Exogenous Ion Channels

Rezensionen

...an informative book that I would find a useful addition to my book shelf. I suspect that my copy will soon be stolen by a graduate student-always a good test of a book. -Trends in Neurosciences

"...an absolute pleasure from start to finish..." - Today's Life Science