Protocols of Actinomycetes

Microbiology to Gene editing
Herausgeber: Karthik, L.

• Gebundenes Buch

Produktbeschreibung

This book will cover basics to recent protocols for drug discovery from actinobacteria. It will be helpful for the pharmaceutical industries and researchers to develop new antibiotics from actinobacteria and would be used in support of future research in drug discovery.

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Produktdetails

• Verlag: Taylor & Francis Ltd
• Seitenzahl: 354
• Erscheinungstermin: 29. November 2024
• Englisch
• Abmessung: 254mm x 178mm
• ISBN-13: 9781032504230
• ISBN-10: 1032504234
• Artikelnr.: 71183633

Autorenporträt

L. Karthik has an extensive expertise in Actinobacteria, Synthetic biology and Drug Discovery Strategies and is presently working as a Project Leader, Synthetic Biology at Salem Microbes Pvt. Ltd, Salem, India. He is Top 2% of all scientists in the world (the Stanford University report published by Elsevier on 10th October 2022&2023). He is an elected Fellow member of Linnean Society of London. He did first postdoctoral training at the Shanghai Jiao Tong University, China under the guidance of eminent scientist Prof. Zhiyong Li. Further he has completed his second postdoctoral fellowship in ECUST, Shanghai, China under the guidance of eminent scientist Prof. Lixin Zhang. He received his Ph.D. degree in Microbiology from the VIT University in 2013. He has published around 75 research papers (h index-26), 5 Books and 6 patents and 6 products commercialized. He is a communication committee member of International Chemical Biology Society, USA and President of Society of Chemical and Synthetic biology, India. In 2016, he got Prestigious "Young Scientist Award" from Indian Association of Applied Microbiologists for his outstanding research work. and got Best Research scholar award-2012 from VIT University. He got 200 young scientist-2012 fellowship (DST) to attend international conference. He got Best VIT ALUMNI AWARD-2022 from VIT University, TN, India. He is Associate Editor of Frontiers in Microbiology and Review editor of Frontiers in Marine Science and also Associate Editor of Current Chinese Engineering Science Journal (Bentham Science). Recently, he proved COVID-19 activity of Kabasura Kudineer (Siddha Herbal formulation). During COVID-19 wave, this drug saved lot of life. For this contribution, he got COVID warrior award from SEED Trust, TN, India.

Inhaltsangabe

1. Isolation of Actinomycetota by different methods. 2. Recent Traditional
methods for Isolating Diverse Actinobacteria. 3. Isolation of
actinobacteria from caves and limestone habitats. 4. Isolation of
endophytic actinobacteria from different sources. 5. Sample treatment and
Protocols for the cultivation of marine actinomycetes. 6. A protocol for
screening, cultivation, and molecular identification of rare and
slow-growing actinomycetes with biosynthetic gene clusters. 7. Isolation
and Screening of Enzymes from Actinobacteria. 8. RSM-Based Media
Optimization for Enzyme Production By Streptomyces. 9. Protocols for the
Screening, Extraction, and Purification of the Hydrolytic Enzymes from
Marine Actinomycetes. 10. Marine Actinomycetes' Hydrolytic Enzymes: A
structural characterization.11. Isolation of Beta-Glucanase Encoding Genes
from Actinobacterial Genus Streptomyces Spp. and its Overexpression in E.
coli Heterologous System. 12. Isolation and Screening of Enzyme Inhibitors
from Marine Actinobacteria. 13. Screening of amylase and lipase enzymes
inhibitors from Actinomycetota. 14. Screening and Characterization of
Carotinoids Pigment from the Actinobacteria. 15. Screening of Biosurfactant
activity of Actinobacteria. 16. Traditional Screening of Actinomycetes for
Antibacterial, Antifungal and Antiviral Properties. 17. Isolation and
screening of actinobacteria with antibacterial activity and its production
through shake flask culture method. 18. Screening of Antimycobacterial
property of actinobacteria. 19. Screening actinomycetes for novel
antimycobacterial and anti-cancer molecules. 20. In vitro antioxidant
protocols for Actinobacteria. 21. Protocol for Antileishmanial activity
using marine Actinobacteria. 22. Shining a Light on the Potent
Anti-Malarial Activity actinomycete extract: A Comprehensive In Vitro and
In Vivo Evaluation. 23. Screening of marine actinobacteria for postprandial
anti-diabetic activity In-vitro and In -vivo Methods. 24. Exploring the
Larvicidal Activity of actinomycetes Extracts. 25. Evaluation of
Actinobacteria's antibiofilm properties using qualitative and quantitative
methods. 26. Microplastic degradation by Actinobacteria. 27. Antibacterial
and Antibiofilm activity of biosynthesized nanoparticles from
Actinomycetes: An alternative, environmental-free, sustainable approach.
28. Extraction and purification of rapamycin from Streptomyces
hygroscopicus. 29. Isolation and Identification of Squalene from the ethyl
acetate extract of Streptomyces sp. VITGV156 (MCC 4965). 30. Docking and
ADME studies. 31. Methods and protocols for the extraction of the genomic
DNA, gene cloning, and heterologous expression from the actinomycetes of
extreme habitats. 32. Activation of silent genes in actinomycetes by
different methods. 33. Multimodal Spectroscopy Based Proteomics and
Metabolomics Approach to Explore Actinomycetes. 34. Isolation of DNA and
RNA from actinobacteria. 35. Extraction of DNA from Actinomycetota and
amplification of the 16S rRNA gene. 36. Optimization of colony PCR for the
identification of actinobacteria by sequencing of the 16S rDNA. 37.
Identification of actinomycetes by polyphasic taxonomy. 38. Phylogenetic
Analysis of Actinomycetes. 39. Genetic profiling of actinobacterial genomes
through polymerase chain reaction based techniques. 40. In Silico Analysis
of Phylogenetic Relationship and Non-synonymous Polymorphisms in
Actinobacteria. 41. Isolation of metagenomic DNA and data analysis. 42.
Practical Methodology of Illumina Sequencing Investigating Actinobacteria
Genomes. 43. Digital DNA-DNA hybridization for the identification of
Actinomycetota species. 44. antiSMASH: Genome-mining and genome-wide
analysis of gene clusters in Actinopolyspora species. 45. Transcriptome
Analysis of Actinomycetes RNA Sequencing data: A bioinformatics protocol
for beginners. 46. Single-cell RNA sequencing of the actinomycetes. 47.
Preparation of Streptomyces RNA for transcriptomics. 48. A protocol for
CRISPR-Cas9 mediated knockout construction in Actinobacteria. 49. Design of
gRNA for DNA-directed RNA polymerase beta subunit (rpoB) gene in
Actinomycete. 50. Acute and Chronic Toxicity of Bioactive Compounds from
Actinobacteria. 51. Production, extraction, purification and
characterization of bioactive derivatives of phthalate ester from Marine
Streptomyces sp.VITJS4