Trauma, caries, and clinical procedures can all cause exposure of vital pulp tissue that severely hampers the function and life span of the tooth. Pulpotomy of the deciduous teeth is not only the most frequent endodontic treatment performed on children, but also the most controversial. Based on the amputation of the pulp chamber and the conservation of the inflammation-free root canals, the clinical results can be good, depending on the materials used. This study was conducted to evaluate and compare the clinical, radiographic and histological effect of Formocresol versus Enamel Matrix Derivative on pulpotomized human primary dentition