This book is about the basic methods of purification and characterization of Amidase enzyme isolated from Bacillus sp., APB-6. Using simple language and concepts, this book will enable you to understand the basic concepts of purification, characterization and applications of enzyme, Amidase. It contains the detailed step-wise procedure of purifying any enzyme and characterization of purified enzyme.Further the amino acid sequencing of purified enzyme and postsynthetic modifications of an enzyme could be done by using protein engineering technique.