Sharon Marsh (ed.)

Pyrosequencing Protocols

Herausgegeben:Marsh, Sharon

• Gebundenes Buch

Produktbeschreibung

Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.

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Produktdetails

• Methods in Molecular Biology 373
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 12016358, 978-1-58829-645-0
• 2007 edition
• Seitenzahl: 196
• Erscheinungstermin: 21. November 2006
• Englisch
• Abmessung: 234mm x 160mm x 21mm
• Gewicht: 476g
• ISBN-13: 9781588296450
• ISBN-10: 1588296458
• Artikelnr.: 21325664

Autorenporträt

Sharon Marsh, Washington University School of Medicine, St. Louis, MO, USA

Inhaltsangabe

The History of Pyrosequencing®.- Pyrosequencing® Applications.- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing®.- Pyrosequencing®.- Universal Primer Applications for Pyrosequencing®.- Allele Quantification and DNA Pooling Methods.- Multiplex Pyrosequencing® for DNA Variation Analysis.- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology.- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria.- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci.- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands.- Gene Copy Number Detection in Animal Studies.- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing®.- Pyrosequencing® Genotype Storage Techniques.