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Aim of the present work was to carry out pharmacoepidemiological survey of some marketed polyherbal antidiabetic formulations through the questionnaires evaluating the views of doctors, patients, pharmacists and herbal industries. We then evaluated some phytochemical and analytical quality control parameters for some selected herbal preparations and developed HPTLC methods for quantitative estimation of biomarkers like curcumin, charantin and swetiamarin in some polyherbal formulations like Diabecon® (The Himalaya Drug Company, Bangalore), Mersina® (J & J Dechane Lab. Pvt. Ltd., Hyderabad) and Madhuripu® (LVG, Ahmedabad). The formulation Mersina was finally also evaluated pharmacologically in diabetic patients.