This Study includes quantitative estimation of some essential trace elements from the natural sources like well, bore well and river of different regions. For the study, water samples were collected from natural sources which includes well, bore-well and river from specified regions for determination of essential trace elements which includes calcium, zinc, copper, chloride and nitrate using UV/VIS spectroscopy, titrimetry and other evaluation methods which includes microbial testing and performed as Per Bureau of Indian Standard (BIS).