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In summary Series of new 2-(2-chlorophenyl)quinoline-4-carboxylic acid-based thiosemicarbazide were synthesized in excellent yields. Synthesized derivatives were characterized by spectral studies 1H NMR, 13C NMR, mass spectrometry, IR analysis, elemental analyses and physical studies of Melting points. All the newly synthesized compounds were screened for biological activity and the results showed good activity compared to the standard drug.