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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Radioimmunoassay (RIA) is a very sensitive technique used to measure concentrations of antigens (for example, hormone levels in the blood) without the need to use a bioassay. Although the RIA technique is extremely sensitive and extremely specific, it requires specialized equipment and is costly. It also requires special precautions, since radioactive substances are used. Therefore, today it has been largely supplanted by the ELISA method, where the antigen-antibody…mehr

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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Radioimmunoassay (RIA) is a very sensitive technique used to measure concentrations of antigens (for example, hormone levels in the blood) without the need to use a bioassay. Although the RIA technique is extremely sensitive and extremely specific, it requires specialized equipment and is costly. It also requires special precautions, since radioactive substances are used. Therefore, today it has been largely supplanted by the ELISA method, where the antigen-antibody reaction is measured using colorimetric signals instead of a radioactive signal. The RAST test (radioallergosorbent test) is an example of radioimmunoassay. It is used to detect the causative allergen for an allergy.