Rapid Cycle Real-Time PCR ¿ Methods and Applications
Genetics and Oncology
Herausgegeben von Dietmaier, W.; Wittwer, Carl; Sivasubramanian, N.
Rapid Cycle Real-Time PCR ¿ Methods and Applications
Genetics and Oncology
Herausgegeben von Dietmaier, W.; Wittwer, Carl; Sivasubramanian, N.
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Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the…mehr
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- Produktdetails
- Verlag: Springer / Springer Berlin Heidelberg / Springer, Berlin
- Artikelnr. des Verlages: 80105486, 978-3-642-63965-4
- 2002
- Seitenzahl: 216
- Erscheinungstermin: 30. September 2011
- Englisch
- Abmessung: 242mm x 193mm x 12mm
- Gewicht: 430g
- ISBN-13: 9783642639654
- ISBN-10: 3642639658
- Artikelnr.: 40735358
- Verlag: Springer / Springer Berlin Heidelberg / Springer, Berlin
- Artikelnr. des Verlages: 80105486, 978-3-642-63965-4
- 2002
- Seitenzahl: 216
- Erscheinungstermin: 30. September 2011
- Englisch
- Abmessung: 242mm x 193mm x 12mm
- Gewicht: 430g
- ISBN-13: 9783642639654
- ISBN-10: 3642639658
- Artikelnr.: 40735358