Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the…mehr
Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!
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Inhaltsangabe
From the contents: Rapid Genotyping of 2-bp and 9-bp Deletion Mutations by LightCycler - Rapid Screening for Five Major Cystic Fibrosis Mutations by Melting Peak Analysis Using Fluorogenic Hybridization Probes - Genotyping of the Methionnine-Valine Polymorphism at Codon 129 of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled Hybridization Probes - SYBR Green I Genotyping of Trinucleotide Repeat Loci by Tm - Limitations of Melting Curve Analysis Using SYBR Green I - Fragment Differentiation and Mutation Detection in the CFTR-Gene - Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach - Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene - Rapid Quantitative Detection of Free Cancer Cells in the Peritoneal Cavity of Gastric Cancer Patients with Real-Time CEA RT-PCR Using Hybridization Probes - Expression Analysis of Telomerase-Genes hTERT and hTR by quantitative PCR on the LightCycler
From the contents: Rapid Genotyping of 2-bp and 9-bp Deletion Mutations by LightCycler - Rapid Screening for Five Major Cystic Fibrosis Mutations by Melting Peak Analysis Using Fluorogenic Hybridization Probes - Genotyping of the Methionnine-Valine Polymorphism at Codon 129 of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled Hybridization Probes - SYBR Green I Genotyping of Trinucleotide Repeat Loci by Tm - Limitations of Melting Curve Analysis Using SYBR Green I - Fragment Differentiation and Mutation Detection in the CFTR-Gene - Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach - Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene - Rapid Quantitative Detection of Free Cancer Cells in the Peritoneal Cavity of Gastric Cancer Patients with Real-Time CEA RT-PCR Using Hybridization Probes - Expression Analysis of Telomerase-Genes hTERT and hTR by quantitative PCR on the LightCycler
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