Rare-earth element biochemistry: Methanol dehydrogenases and lanthanide biology

Herausgegeben:Cotruvo, Joseph A.

• Gebundenes Buch

Produktbeschreibung

Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more.

Produktdetails

• Methods in Enzymology Volume 650
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0076-6879(21)X0006-7
• Englisch
• Abmessung: 16mm x 152mm x 229mm
• Gewicht: 560g
• ISBN-13: 9780128238561
• Artikelnr.: 67579421

Autorenporträt

Joseph A. Cotruvo, Jr. is Assistant Professor, Louis Martarano Career Development Professor, Department of Chemistry
Pennsylvania State University, PA, USA

Inhaltsangabe

Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs



Tomoyuki Nakagawa



Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor



Eric Schelter



Activity assays of methanol dehydrogenases



Lena Daumann



Expression, purification and properties of enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, XoxG4



Ludmila Chistoserdova



Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1



Norma Cecilia Martinez-Gomez



Heterologous expression, purification, and characterization of proteins involved in lanthanide utilization



Joseph A. Cotruvo Jr.



Transposon mutagenesis for methylotrophic bacteria using Methylorubrum extorquens AM1 as a model system



Elizabeth Skovran



Metals and Methylotrophy: Global Gene Expression Studies



Marina G. Kalyuzhnaya



Mass spectrometry-based approaches to study lanthanides in the phyllosphere



Julia A. Vorholt



Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph "Methylotuvimicrobium buryatense" 5GB1C



Joseph Douglas Groom Jr.