R. A. J. Challiss (ed.)

Receptor Signal Transduction Protocols

Herausgeber: Challiss, R. A. J.

• Gebundenes Buch

Produktbeschreibung

An outstanding collection of state-of-the-art methods from leading experts for interrogating ligand-receptor and receptor-effector interactions. The methods emphasize the regulation of G protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G proteins, and approaches to studying how receptor expression and function is regulated. The easily reproducible protocols include chimeric receptor construction, antibody generation, transient and stable transfection methods, in situ hybridization, as well as immunocytochemistry, functional assays of G protein function, antisense methods for defining receptor-effector coupling pathways, and many more.

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Produktdetails

• Methods in Molecular Biology 83
• Verlag: Springer, Berlin
• 1997.
• Seitenzahl: 275
• Erscheinungstermin: 8. Mai 1997
• Englisch
• Abmessung: 237mm x 160mm x 24mm
• Gewicht: 608g
• ISBN-13: 9780896034952
• ISBN-10: 089603495X
• Artikelnr.: 23140092

Inhaltsangabe

Methods for Characterizing Receptors.
Radioligand
Binding Methods for Membrane Preparations and Intact Cells.
Site
Directed Mutagenesis and Chimeric Receptors in the Study of Receptor
Ligand Binding.
Approaches to the Stable Transfection of G Protein
Coupled Receptors.
Methods for Transient Expression of Hetero
Oligomeric Ligand
Gated Ion Channels.
The Generation of Receptor
Selective Antibodies.
In Situ Hybridization.
lmmunocytochemical Methods for Investigating Receptor Localization.
Methods for Studying Receptor Coupling Mechanisms.
Measurement of Agonist
Stimulated [35S]GTP?S Binding to Cell Membranes.
Autoradiographic Visualization in Brain of Receptor
G Protein Coupling Using [35S]GTP?S Binding.
Agonist
Induced High
Affinity GTP Hydrolysis as an Index of Receptor
Mediated G Protein Activation in Mammalian Brain Membranes.
Use of Random
Saturation Mutagenesis to Study Receptor
G Protein Coupling.
Identification and Quantitation of G Protein ?
Subunits.
Covalent Modification of G Proteins by Affinity Labeling.
Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection.
Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identify G Protein Heterotrimers Activating Phospholipase C.
Use of Antisense
Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons.
Methods for Investigating the Regulation of Receptor Function.
Protocols Employed in the Investigation of G Protein
Coupled Receptor Phosphorylation.
Assay of G Protein
Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation.
Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cells.
Regulation of ReceptorExpression.