Replicative Cessation & DNA Damage in Human Fibroblast under Stress - Khalil, Sahar;Hammouda, Gehad;Hosny, Somaya
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Aging can be viewed partly as a degenerative process dictated by free radical reactions. Human diploid fibroblasts (HDFs) lose the capacity to proliferate and enter a state termed replicative senescence after a finite number of population doublings in vitro. Presenescent human fibroblasts, when treated with a sub-lethal dose of H2O2, enter long term growth arrest resembling replicative senescence. H2O2 induced growth arrest; p53 protein expression and the effect of vitamin C as an antioxidant were determined in this study.

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Produktbeschreibung
Aging can be viewed partly as a degenerative process dictated by free radical reactions. Human diploid fibroblasts (HDFs) lose the capacity to proliferate and enter a state termed replicative senescence after a finite number of population doublings in vitro. Presenescent human fibroblasts, when treated with a sub-lethal dose of H2O2, enter long term growth arrest resembling replicative senescence. H2O2 induced growth arrest; p53 protein expression and the effect of vitamin C as an antioxidant were determined in this study.
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Autorenporträt
Name: Sahar Khalil Abdel-Gawad,MD, DHPE.Lecturer of Histology & Cell Biology, Faculty of Medicine, Suez Canal University (FOM/SCU), Egpyt.Has a Diploma Degree in Health Professions Education (DHPE) in 2010 from Suez Canal University (FOM/SCU), Egpyt.