Research Methods in Neurochemistry

Volume 3
Herausgegeben:Marks, Neville

• Broschiertes Buch

Produktbeschreibung

With the continued rapid expansion of neurochemical research, there has been no shortage of new developments in methodology for this third volume of Research Methods in Neurochemistry. As in previous volumes we have again tried to provide some balance in the subjects represented. The wisdom of this policy may be questioned since it can lead to delay in publica tion, but there are many approaches to the chemical study of the nervous system and a methods book needs to stand on its own as well as be part of a series. In one respect, however, the present volume departs from this policy, in that we have included two chapters on micromethods for analyzing amines and amino acids, both giving special emphasis to dansylation techniques. These chapters are complementary and we feel justified in publishing them in one volume in view of the importance of such micromethods for the study of neural systems. At the other end of the scale, particular attention may be drawn to the chapter by D. D.Gilboe and colleagues describing their remarkable procedures for studying metabolism in the isolated canine brain. We were fortunate also in persuading S. S. Oja to extend the general prin ciples of transport systems he described in Volume 2 to amino acids in brain slices. In addition, there are the usual chapters on components of neural tissues, which once again we have found convenient to divide into enzymes, macromolecules, and other constituents.

Produktdetails

• Verlag: Springer / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4613-4460-5
• Softcover reprint of the original 1st ed. 1975
• Seitenzahl: 492
• Erscheinungstermin: 5. November 2011
• Englisch
• Abmessung: 229mm x 152mm x 27mm
• Gewicht: 714g
• ISBN-13: 9781461344605
• ISBN-10: 1461344603
• Artikelnr.: 39617140

Inhaltsangabe

Section I Properties of Intact Neural Tissues.- 1 Use of the Isolated Canine Brain in Studies of Cerebral Metabolism, Metabolite Transport, and Cerebrovascular Physiology.- 2 Axoplasmic Transport.- 3 Transport of Amino Acids in Brain Slices.- 4 Glycine Enzymes and Uptake Systems.- Section IIA Components of Neural Tissues: Enzymes.- 5 Assay and Purification of Brain Monoamine Oxidase.- 6 Acetylcholinesterase.- 7 Radiochemical Assays for Choline Acetyltransferase and Acetylcholinesterase.- 8 Phosphate-Activated Glutaminase in Brain.- Section IIB Components of Neural Tissues: Lipids, Proteins, and Polylipids.- 9 Analysis of Phospholipids by Sequential Chemical Degradation.- 10 Analysis of Free and Esterified Fatty Acids in Neural Tissues Using Gradient-Thickness Thin-Layer Chromatography (GT-TLC).- 11 Methods for Studying Protein Phosphorylation in Cerebral Tissues.- 12 Assays of Hypothalamic Releasing and Inhibiting Hormones.- 13 Structure-Activity Relationship of LH and FSH Releasing Hormone.- Section IIC Components of Neural Tissues: Amino Acids.- 14 Assay Procedures for Polyamines and GABA in Animal Tissues with Special Reference to Dansylation Methods.- 15 The Use of Dansyl-Chloride for the Detection of Amino Acids and Serotonin in Nervous Tissue.