In this work, three types of nanocomposite materials, Ni50Co50(OH)2/BOX PtNiCu, and Fe(OH)x/HCO3-/FTO were prepared, and two nanocomposite materials, PtNiCu and Ni50Co50(OH)2/BOX were constructed. The relationship between the morphology, composition, size and electrocatalytic performance of the nanocomposite material and the sensing interface and the response performance of the sensor was studied, and the electrochemistry for the detection of glucose, catechol and hydroquinone was established. The Fe(OH)x/HCO3-/FTO was used in the research of electrolytic water splitting. This research can provide a new method for the analysis of glucose and dihydroxy benzene isomers, and can also enrich the phase research of electrocatalytic water splitting.