Not much work has been done so far on leaf blight disease of barley with respect to molecular and biochemical aspects related to resistance. Hence, an attempt was made for a systematic study of the various resistance factors (molecular and biochemical) which attribute to the resistance of the barley genotypes, which would help us to know the nature of resistance present in different genotypes and to evolve a suitable resistant genotypes against this disease. Keeping this in view, the present investigation was under taken with the following objectives: 1. Biochemical factors imparting resistance. 2. Role of isozymes in resistance. 3. DNA finger printing of leaf blight resistant and susceptible genotypes.