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In recent years, the development of surgical means of sperm collection, in particular testicular biopsy, has made it possible for azoospermic patients to have their own offspring through the microinjection of a single sperm into the oocyte cytoplasm (ICSI). The objective of this work was to compare the results of ICSI with frozen-thawed testicular sperm. 70 azoospermic patients, divided into two comparable groups, a first group of 28 patients with fresh sperm from a BT synchronous with ovarian puncture and a second group of 42 patients with frozen-thawed sperm from a testicular biopsy delayed at ovarian puncture.There was no statistically significant difference between the first and second groups in terms of fertilization rate, cleavage rate (87% versus 86%; p=0.86), top embryo rate (73.5% versus 73.3%; p=0.96), mean number of blastocysts obtained (2.2 versus 2.15; p=0.96), implantation rate (23.7% versus 19.4%; P=0.53) and clinical pregnancy rate (43% versus 50%; p=0.62). Testicular sperm freezing does not seem to influence the results of ICSI and is therefore the alternative to the other methods.