Herve Bazin

Revival

Rat Hybridomas and Rat Monoclonal Antibodies (1990)

• Broschiertes Buch

Produktbeschreibung

The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.

---

Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.

Produktdetails

• Verlag: CRC Press
• Seitenzahl: 534
• Erscheinungstermin: 28. Januar 2019
• Englisch
• Abmessung: 254mm x 178mm x 29mm
• Gewicht: 993g
• ISBN-13: 9781138561649
• ISBN-10: 1138561649
• Artikelnr.: 56894733

Autorenporträt

Herve Bazin

Inhaltsangabe

Introduction
Part I IMMUNOCYTOMAS in LOUVAIN (LOU) RATS.
Chapter 1
Rat Immuoglobulins.
Chapter 2
The LOUVAIN (LOU) Rats.
Chapter 3
Rat Immunocytomas (IR).
Part II PRODUCTION of RAT-RAT HYBRIDOMAS.
Chapter 4
Fusion Cell Lines.
Chapter 5
Immunization of Rats.
Chapter 6.I
Fusion Procedure.
Chapter 6.II
Rat Hybridoma Prodcution: Scanning electron Microscopy of a Fusion
Experiment
Chapter 7
Selective Growth of Hybrids.
Chapter 8
Cloning.
Chapter 9.I
Screening of Monoclonal Antibodies Directed Against Soluble Antigens
Chapter 9.II
Screening of Hybrid Supernatants for Monoclonal Antibodies Against Cellular
Antigens
Chapter 10
Isotyping of Rat Monoclonal Antibodies.
Chapter 11
Adaptation to In Vivo Culture. Freezing and Thawing.
Chapter 12
Freezing and Thawing
Part III PRODUCTION, PURIFICATION, FRAGMENTATION and LABELING of MONOCLONAL
ANTIBODIES.
Chapter 13
In Vitro Production of Rat Monoclonal Antibodies.
Chapter 14
In Vivo Production of Rat Monoclonal Antibodies.
Chapter 15
Purification of Rat Monoclonal Antibodies from Ascitic Fluid - Serum or
Culture Supernatant.
Chapter 16
Quality Control of Purified Antibodies.
Chapter 17
Fragmentation of Rat Monoclonal Antibodies.
Chapter 18
Labeling of Monoclonal Antibodies.
Part IV EXAMPLES of PRODUCTION and CHARACTERIZATION of RAT-RAT HYBRIDOMAS
AGAINST VARIOUS Ag.
Chapter 19.I
Rat Monoclonal Antibodies Against Murine Immunoglobulins.
Chapter 19.II
Allotype Specific Monoclonal Antibodies to Rat Kappa Light Chain.
Chapter 19.III
Application of Rat Hybridomas to Studies on Ocular Immunobiology.
Chapter 19.IV
Rat Monoclonal Antibodies Against Rabbit Immunoglobulin.
Chapter 19.V
Preparation of Anti-Human IgG Monoclonal Antibodies Using Rat x Rat
Hybridoma System.
Chapter 19.VI
Rat Monoclonal Antibodies Against Human Immunoglobulin.
Chapter 19.VII
Production and Characterization of Rat Monoclonal Antibodies Directed
Against Human Apolipoprotein B.
Chapter 19.VIII
Studies on Rat-Rat Hybridoma Technique and its Application to Obtain Rat
Monoclonal Antibodies Anti-Horse-radish Peroxidase.
Chapter 19.IX
Rat-Rat and Rat-Mouse Hybridomas Secreting Rat Monoclonal Anti-Horse-radish
Peroxidase.
Chapter 19.X
LOU Rat Monoclonal Antibodies Against Xenopus Vitellogenin.
Chapter 19.XI
Production and Characterization of Rat-Rat Hybridomas Against DNP-Hapten.
Rat x Rat Hybridomas Against Cellular Antigens.
Chapter 20.I
Rat Monoclonal Antibodies Against Human Leukocyte Differentiation Antigens.
Chapter 20.II
Rat Monoclonal Antibodies Specific for T Lymphocytes.
Chapter 21.I
Rat Monoclonal Antibodies Against Streptococcus mutans and Bacteroides
gingivalis.
Chapter 21.II
A Specific Rat Monoclonal Antibody Against Yersinia enterocolitica
Serogroup 0:8.
Chapter 21.III
Production of Rat Hybridoma Directed Against Yersinia enterocolitica.
Chapter 22
Rat Monoclonal Antibodies Against Viral Antigens.
Chapter 23
Contribution of Rat Monoclonal Antibodies to the Study of Schistosoma
mansoni Infections.
Part V SPECIFIC USES and PROPERTIES of RAT MONOCLONAL ANTIBODIES.
Chapter 24.I
Mouse Immunoglobulin Isotyping.
Chapter 24.II
Humoral Immune Suppression of Mice by Monoclonal Rat Anti-Mouse µHeavy
Chain Antibodies.
Chapter 24.III
Rat Monoclonal Antibodies Against Mouse IgE.
Chapter 24.IV
Purification of Mouse Monoclonal Antibodies by Immunoaffinity
Chromatography.
Chapter 24.V
Screening of Mouse Monoclonal Antibodies.
Chapter 24.VI
Use of Rat Monoclonal Antibodies in Immunohistochemistry Investigations.
Chapter 24.VII
Use of Rat-Rat Monoclonal Antibodies Labeled with Fluorochrome.
Chapter 25.I
Use of Rat Monoclonal Anti-Rat Allotype Antibodies in Immunohistoperoxidase
Technique and ELISA.
Chapter 25.II
Use of Anti-Rat Kappa Light Chain Allotype Monoclonal Antibodies for the
Quantification of Rat Monoclonal Antibodies in Ascitic Fluid or Serum.
Chapter 26
Extracorporeal Removal of Arteriogenic Particles by Use of Rat Monoclonal
Antibody Coupled to Sepharose as Specific Immunosorbent.
Chapter 27
Antibacteria Antigens, Especially Streptococcus mutans.
Chapter 28
Mercury-Induced Autoimmunity Production of Monoclonal Autoantibodies.
Chapter 29
Rat Monoclonal Antibodies to Plant Pathogens.
Chapter 30.I
Rat Monoclonal Antibodies for Complement-Mediated and Cell-Medicated
Cytolysis.
Chapter 30.II
Rat Antibodies and K Cells.
Chapter 31
Absence of Viral Particles in Rat Hybridomas - An Ultrastructural Study.
Chapter 32
Role of CODATA-HDB in the Centralization and Distribution of Information
Concerning the Rat Hybridomas.
Chapter33
List of Monoclonal Antibodies.