Robert E. Farrell

RNA Methodologies

A Laboratory Guide for Isolation and Characterization

• Broschiertes Buch

Produktbeschreibung

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center Includes classic and contemporary techniques Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center Includes classic and contemporary techniques Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Produktdetails

• Verlag: Academic Press
• 3rd edition
• Seitenzahl: 688
• Englisch
• Abmessung: 229mm
• Gewicht: 1100g
• ISBN-13: 9780122496967
• ISBN-10: 0122496965
• Artikelnr.: 20803802

Inhaltsangabe

Chapter 1: RNA and the Cellular Biochemistry Revisited Chapter 2: Transcription and the Organization of Eukaryotic Genes Chapter 3: Messenger RNA Chapter 4: Resilient Ribonucleases Chapter 5: RNA Isolation Strategies Chapter 6: The Truth About Tissues Chapter 7: Isolation of Polyadenylated RNA Chapter 8: Quality Control for RNA Preparations Chapter 9: Dot Blot Analysis Chapter 10: Electrophoresis of RNA Chapter 11: Photodocumentation and Image Analysis Chapter 12: Northern Analysis Chapter 13: Nucleic Acid Probe Technology Chapter 14: Practical Nucleic Acid Hybridization Chapter 15: Principles of Detection Chapter 16: Quantification of Specific mRNAs by Nuclease Protection Chapter 17: Analysis of Nuclear RNA Chapter 18: cDNA Synthesis Chapter 19: RT-PCR Chapter 20: Quantitative PCR Techniques Chapter 21: Transcript Subtraction Methods Chapter 22: mRNA Differential Display Chapter 23: High-Throughput Analysis of Gene Expression Chapter 24: RNA Interference: Targeted Gene Silencing Chapter 25: Genomes, Transcriptomes, Proteomes, and Bioinformatics Chapter 26: An RNA Paradigm Epilogue: A Few Pearls of Wisdom Appendix A: Maintaining Complete and Accurate Records Appendix B: Useful Stock Solutions for the Molecular Biologist Appendix C: Phenol Preparation Appendix D: Disposal of Ethidium Bromide and SYBR Green Solutions Appendix E: DNase I Removal of DNA from an RNA Sample Appendix F: RNase Incubation to Remove RNA from a DNA Sample Appendix G: Deionization of Formamide, Formaldehyde, and Glyoxal Appendix H: Silanizing Centrifuge Tubes and Glassware Appendix I: Centrifugation as a Mainstream Tool for the Molecular Biologist Appendix J: Trypsinization Protocol for Anchorage-Dependent Cells Appendix K: Isolation of High-Molecular-Weight DNA by Salting-Out Appendix L: RNA Isolation from Plant Tissue Appendix M: Electrophoresis: Principles, Parameters, and Safety Appendix N: Polyacrylamide Gel Electrophoresis Appendix O: Selected Suppliers of Equipment, Reagents, and Services Appendix P: Useful SI Units Appendix Q: Common Abbreviations Appendix R: Trademark Citations Glossary Index Chapter 1: RNA and the Cellular Biochemistry Revisited Chapter 2: Transcription and the Organization of Eukaryotic Genes Chapter 3: Messenger RNA Chapter 4: Resilient Ribonucleases Chapter 5: RNA Isolation Strategies Chapter 6: The Truth About Tissues Chapter 7: Isolation of Polyadenylated RNA Chapter 8: Quality Control for RNA Preparations Chapter 9: Dot Blot Analysis Chapter 10: Electrophoresis of RNA Chapter 11: Photodocumentation and Image Analysis Chapter 12: Northern Analysis Chapter 13: Nucleic Acid Probe Technology Chapter 14: Practical Nucleic Acid Hybridization Chapter 15: Principles of Detection Chapter 16: Quantification of Specific mRNAs by Nuclease Protection Chapter 17: Analysis of Nuclear RNA Chapter 18: cDNA Synthesis Chapter 19: RT-PCR Chapter 20: Quantitative PCR Techniques Chapter 21: Transcript Subtraction Methods Chapter 22: mRNA Differential Display Chapter 23: High-Throughput Analysis of Gene Expression Chapter 24: RNA Interference: Targeted Gene Silencing Chapter 25: Genomes, Transcriptomes, Proteomes, and Bioinformatics Chapter 26: An RNA Paradigm Epilogue: A Few Pearls of Wisdom Appendix A: Maintaining Complete and Accurate Records Appendix B: Useful Stock Solutions for the Molecular Biologist Appendix C: Phenol Preparation Appendix D: Disposal of Ethidium Bromide and SYBR Green Solutions Appendix E: DNase I Removal of DNA from an RNA Sample Appendix F: RNase Incubation to Remove RNA from a DNA Sample Appendix G: Deionization of Formamide, Formaldehyde, and Glyoxal Appendix H: Silanizing Centrifuge Tubes and Glassware Appendix I: Centrifugation as a Mainstream Tool for the Molecular Biologist Appendix J: Trypsinization Protocol for Anchorage-Dependent Cells Appendix K: Isolation of High-Molecular-Weight DNA by Salting-Out Appendix L: RNA Isolation from Plant Tissue Appendix M: Electrophoresis: Principles, Parameters, and Safety Appendix N: Polyacrylamide Gel Electrophoresis Appendix O: Selected Suppliers of Equipment, Reagents, and Services Appendix P: Useful SI Units Appendix Q: Common Abbreviations Appendix R: Trademark Citations Glossary Index

Rezensionen

Praise for the third edition "This third edition of RNA Methodologies is an updated version of a laboratory guide edited for the first time in 1993; it represents an exhaustive collection of tried, tested, and optimized protocols for isolation and chracterization of RNA. The third edition of RNA Methodologies is a well-documented guide for any researcher who has to manage RNA studies. Each chapter of this book provides a rationale to help in the decision-making process...The inclusion of troubleshooting hints and annotations based on personal experience are provided to improve the method(s). This laboratory guide offers protocols on traditional and new technologies, with a focus on how to use the expedient technique for achieving an experimental goal in the study of gene expression." --Dr. Patrick Pagesy at INSERM for HEMOGLOBIN Praise for the third edition "This third edition of RNA Methodologies is an updated version of a laboratory guide edited for the first time in 1993; it represents an exhaustive collection of tried, tested, and optimized protocols for isolation and chracterization of RNA. The third edition of RNA Methodologies is a well-documented guide for any researcher who has to manage RNA studies. Each chapter of this book provides a rationale to help in the decision-making process...The inclusion of troubleshooting hints and annotations based on personal experience are provided to improve the method(s). This laboratory guide offers protocols on traditional and new technologies, with a focus on how to use the expedient technique for achieving an experimental goal in the study of gene expression." --Dr. Patrick Pagesy at INSERM for HEMOGLOBIN