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The functions of bacterial surface Extracellular polymeric substances (EPS) and Lipopolysaccharides (LPS) on bacterial adhesion to quartz sand surfaces were examined using techniques such as packed-column experiment, Quartz Crystal Microbalance with Dissipation (QCM-D), potentiometric titration,zeta potential etc.. In addition,the transport and adhesion of Total Coliform, Escherichia coli and Enterococci under different climatic conditions at Bradford Beach,Milwaukee was explored.