RP-HPLC Method Development and Validation - Hechhu, Ramana;Balla, Tamrat Balcha;Vasanthi, R.
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High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of Thiocolchicoside and Lornoxicam was done by RP-HPLC. The Phosphate buffer was pH 3.0 and the mobile phase was optimized with consists of Methanol: Phosphate buffer mixed in the ratio of 70:30 % v/ v. Inertsil C18 column C18 (4.6 x 150mm, 5mim) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 260 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. the…mehr

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Produktbeschreibung
High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of Thiocolchicoside and Lornoxicam was done by RP-HPLC. The Phosphate buffer was pH 3.0 and the mobile phase was optimized with consists of Methanol: Phosphate buffer mixed in the ratio of 70:30 % v/ v. Inertsil C18 column C18 (4.6 x 150mm, 5mim) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 260 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. the linearity range of Thiocolchicoside and Lornoxicam were found to be from 100-500 mig/ml of Thiocolchicoside and 1-5mig/ml of Lornoxicam . Linear regression coefficient was not more than 0.999.The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 98-102% of Thiocolchicoside and Lornoxicam . LOD and LOQ were found to be within limit.The results obtained on the validation parameters met ICH and USP requirements .it inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application.
Autorenporträt
Dr. RAMANA HECHHU M.Pharmacy & PhD. has 16 years of teaching and research experience in Pharmaceutical Sciences and Published more than 30 research publications in National and International journals of repute. He is currently teaching at School of Pharmacy, College of Health Sciences and Medicine, Wolaita Sodo University, Ethiopia as a Professor.