Sample Preparation Techniques for Soil, Plant, and Animal Samples

Herausgegeben:Micic, Miodrag

• Gebundenes Buch

Produktbeschreibung

This protocol volume will be the first publication to comprehensively cover entire spectrum of molecular biology sample preparation techniques commonly used to extract and isolate nucleic acids, proteins and other molecules of interests, from plants, animals and environmental samples and similar difficult samples. Sample preparation is considered to be the first and most significant bottleneck in the molecular biology lab workflow, and it is very peculiar fact that while there is an estimated $3.6B market (Bcc Research Report ( BIO089A, 2010) in sample preparation consumables and equipment for Genomics, Proteomics, and Epigenetics; and that approximately 180,000 people worldwide are performing sample preparation techniques in the lab daily, there is no single book dealing exclusively with this topic. The proposed book will present an in depth literature review of current state-of-the-art knowledge in all aspects of molecular biology related sample preparations methods, techniquesand applications, and provide a comprehensive collection of practical recipes and advice on the most efficient methods of processing the most common sample types in agriculture, plant science, soil science, environmental science, and animals research. The book will be an essential tool for demystifying time consuming, and often the last remaining bottleneck process in molecular biology sample preparation and will be of special interest to all lab technicians bench scientists, graduate students, postdocs, as well as other senior scientists working with DNA, RNA and Proteins in such a diverse areas ranging from plant, soil, environmental science, animal research, as well as to more applied fields of quality control and law enforcement.

Produktdetails

• Springer Protocols Handbooks
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-3184-2
• 1st ed. 2016
• Seitenzahl: 432
• Erscheinungstermin: 29. Januar 2016
• Deutsch, Englisch
• Abmessung: 241mm x 160mm x 28mm
• Gewicht: 832g
• ISBN-13: 9781493931842
• ISBN-10: 1493931849
• Artikelnr.: 43179906

Autorenporträt

Miodrag "Mickey" Mi¿i¿ , Ph.D., Sc.D., M.T.M., C.S.W.P. is the Department Chair and Professor at the Department of Engineering Design Technology and Department of New Products Development at Cerritos College. He is also a Project Scientist at the BioMEMS Lab, Department of Mechanical and Aerospace Engineering, University of California, Irvine. He is a Visiting Professor at the Faculty of Applied Ecology-Futura at Singidunum University, Belgrade, Serbia. Dr. Mi¿i¿ is a consultant to several life sciences, medical devices, and biotech companies. Prior to his current position at Cerritos College, Dr. Mi¿i¿ was the Vice President of Research and Development at MP Biomedicals LLC (Formerly ICN Biomedicals Inc), Irvine, CA, USA. Earlier in his career, he was a Senior Application Scientist at Veeco Metrology, Santa Barbara, CA, USA. Dr. Mi¿i¿ did his postdoctoral work as a Postdoctoral Research Fellow at Pacifi c Northwest National Laboratories, Richland, WA, USA. He is theauthor of 67 publications in leading scientific journals, with over 1,400 citations. Dr. Mi¿i¿ holds a Ph.D. and M.S. degrees in Chemistry from the University of Miami, Coral Gables, FL, USA. He holds an M.T.M. degree from Washington State University, WA, USA. Further, Dr. Mi¿i¿ holds Sc.D. and M.Sc. degrees in Physical Chemistry; a B.Sc./B.Ed. in Physics and Fundamentals of Engineering from the University of Belgrade, Belgrade, Serbia; and a B.Eng. degree in Mechanical/Naval Architecture Engineering from the Polytechnic Academy of New Belgrade, Belgrade, Serbia. He is also an FAA licensed instrument-rated commercial pilot and a PADI master scuba diver.

Inhaltsangabe

Physical and Chemical Properties of Nucleic Acids.- Sampling for Biostatistics.- Sampling of Microbiological Samples.- Techniques for Nucleic Acid Purifications from Plant, Animal, and Microbial Samples.- Magnetic beads based nucleic acids purification for molecular biology applications.- Systems for Mechanical Sample Lysis and Homogenization for Molecular Biology Applications.- Bead beating offers high performance homogenization for molecular biology downstream processing of tough and difficult samples.- High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and proteins extraction with FastPrep® Systems.- Portable Systems for Sample Lysis and Homogenization.- DNA and RNA extractions from mammalian samples.- Sample preparation for multiplex PCR assays for food and agriculture applications.- Nucleic acid sample preparation from stem cells.- Nucleic Acids Sample Preparation from Teeth/Dental Remains.- Nucleic Acids Sample Preparation for Food Traceability.- Nucleic Acids Sample Preparation for Quantitative and Qualitative GMO analysis.- Nucleic Acids Samples Preparation from Dairy Products and Milk.- Extraction of DNA from Plant Tissue: Review and Protocols.- DNA Extraction from Seeds.- RNA extractions from difficult to prepare and high starch content seeds.- Nucleic Acid and Protein Sample Preparation from Yeasts.- Nucleic acids purification from soil and environmental sources.- Extraction and Purification of Viral Nucleic Acids from Environmental Samples.- Preparation of nucleic acids from marine samples: applications to microbial ecology research.- Nucleic Acid Sample Preparation from Feces and Manure.- Bead-beating sample preparation for nucleic acids isolation from fecal samples.- Methods for extraction and purification of lignin and cellulose from plant tissues.- Separation and purification of glycans out of glycoproteins.- Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional Renilla-prion fusion protein from bacteria.

Rezensionen

"This monograph summarises and presents in an organised single text scattered relevant background information concerning sample preparation for a variety of molecular biology analyses, together with a number of protocols for successful treatment of difficult matrices. This book can be considered a useful practical guide for all those involved in sample preparation within the molecular biology practice, but it is also suitable for newcomers trying to obtain a general, but complete overview of the subject." (Lourde Ramos, Chromatographia, Vol. 79, 2016)