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Schiff's bases named after Hugo Schiff, is a type of chemical compounds containing a carbon-nitrogen double bond as functional group. Schiff bases are versatile ligands which are synthesized from the condensation of primary amines with carbonyl groups and have number of applications as pigments and dyes, catalysts, intermediates in organic synthesis. Schiff bases shows broad rangeof biological activities like antibacterial, antimalarial, antifungal, anti-inflammatory, antiviral, antiproliferative, antitumor activityand antipyretic properties. Because of wide applications of Schiff bases in biology and histochemistry, it can be used for studying cellular content, origin, pathogenesis and diagnosis of head and neck region. Wide spectrum applications of the Schiff base have opened a new era of horizon in the field of research of biological system.They are considered interesting research subject that constantly provides us a platform with new information about newly created compound.This review reflects the essential role of Schiff bases & their modifications for diagnostic & therapeutic purposes.