Schmidtea Mediterranea

Methods and Protocols
Herausgeber: Gentile, Luca

• Broschiertes Buch

Produktdetails

• Methods in Molecular Biology Nr.2680
• Verlag: Springer US
• 2023
• Seitenzahl: 292
• Erscheinungstermin: 12. Juli 2024
• Englisch
• Abmessung: 254mm x 178mm x 16mm
• Gewicht: 554g
• ISBN-13: 9781071632772
• ISBN-10: 1071632779
• Artikelnr.: 71253645

Inhaltsangabe

Building Phylogenies from Transcriptomic Data.
 Isolation and Library Preparation of Planarian piRNAs.
Genome
Wide Analysis of Planarian piRNAs.
Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians.
Colorimetric Whole
Mount In Situ Hybridization in Planarians.
Single Molecule Fluorescent In Situ Hybridization (smFISH) on Whole
Mount Planarians.
Whole
Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea.
Preparing Planarian Cells for High
Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry.
An RNA/DNA
Based Flow Cytometry Approach for Isolating Slow
Cycling Stem Cells.
ACME Dissociation
Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells.
Papain
Based Dissociation of Schmidtea mediterranea Cells.
Live Immunostaining and Flow Cytometry of Schmidtea mediterranea Cells.
Live Imaging in Planarians: Immobilization and Real
Time Visualization of Reactive Oxygen Species.
A Planarian Model System to Study Host
Pathogen Interactions.
TUNEL Staining in Sections of Paraffin
Enabled Planarians.
Quantitative Analysis of Planarian Pigmentation.
mRNA Transfection of S. mediterranea Cells for Luminescence Analysis.