This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells…mehr
This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of peripheral nerve development, cancer, axon degeneration/regeneration, and myelination. Last but not least, part IV outlines protocols for Schwann cell production, collection, labeling and transplantation in the injured peripheral nerve and spinal cord of experimental animals and human subjects.
Authoritative and practical, Schwann Cells: Methods and Protocols aims to aid both experienced and new investigators to make progress in their research endeavors involving Schwann cells.
Isolation of Schwann Cell Precursors from Rodents.- Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies.- Isolation and Expansion of Schwann Cells from Transgenic Mouse Models.- Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers.- Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells.- Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations.- Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies.- Chemical Conversion of Human Fibroblasts into functional Schwann Cells.- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats.- Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells.- Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay.- Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies.- Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis.- Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures.- The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes.- In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia.- A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions.- Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development.- Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs.- 3D Cancer Migration Assay with Schwann Cells.- Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis.- Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration.- The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin.- Analysis of Myelinating Schwann Cells in Human Skin Biopsies.- Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae.- Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin.- Live Imaging of Schwann Cell Development in Zebra Fish.- Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord.- Schwann Cell Transplantation Methods Using Biomaterials.- Viral Transduction of Schwann Cells for Peripheral Nerve Repair.- Intraspinal Delivery of Schwann Cells for Spinal Cord Injury.
Isolation of Schwann Cell Precursors from Rodents.- Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies.- Isolation and Expansion of Schwann Cells from Transgenic Mouse Models.- Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers.- Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells.- Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations.- Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies.- Chemical Conversion of Human Fibroblasts into functional Schwann Cells.- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats.- Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells.- Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay.- Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies.- Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis.- Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures.- The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes.- In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia.- A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions.- Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development.- Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs.- 3D Cancer Migration Assay with Schwann Cells.- Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis.- Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration.- The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin.- Analysis of Myelinating Schwann Cells in Human Skin Biopsies.- Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae.- Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin.- Live Imaging of Schwann Cell Development in Zebra Fish.- Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord.- Schwann Cell Transplantation Methods Using Biomaterials.- Viral Transduction of Schwann Cells for Peripheral Nerve Repair.- Intraspinal Delivery of Schwann Cells for Spinal Cord Injury.
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