A precondition for a positive attitude of children towards nature is the level of their scientific literacy. We point out in this publication the fact that in order to develop the ability to work scientifically, as an essential component of scientific literacy, it is possible to implement this already in pre-primary education. The brain reaches top abilities at the age of five years. It is especially the period of pre-school age which is the most significant for the development of an individual. The aim of this publication is to define the term scientific literacy in the context of pre-primary education, to process the theoretical basis of constructing knowledge in pre-primary education and experimentally verify the effectiveness of the proposed development programme using inquiry-based methods for increasing the levels of scientific literacy of children at a preschool age.