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The compound from the soursop have been successfully isolated and screened for their antimicrobial properties. The soursop was macerated with four different solvents (methanol, ethanol, hexane and distilled water) using maceration technique. Thin layer chromatography (TLC) and column chromatography were used to isolate the crude extract and fractions by using different types of solvent systems. All the crude extracts undergoes Thin layer chromatography (TLC) for isolation and separation of compounds and ethanolic crude extract was selected as the best crude extract. The best solvent system obtained is hexane and ethyl acetate with ratio of 1:3. Then, the compounds in the ethanolic crude extract were further isolated and separated by using column chromatography. TLC analysis was carried out on the fractions collected from column chromatography. The best solvent system for the first column chromatography fraction is ethanol. For the second column chromatography fraction, the best solvent system is methanol and ethyl acetate with ratio of 1:1. Best fraction with one single spot on TLC plate was obtained.
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