Serologic test(s) has not been developed to diagnose abscess disease or to monitor its immune response. This study was conducted to develop a serologic test to diagnose abscess disease by using reference strain of Staphylococcus aureus subsp anaerobius (ATCC 35 844), to monitor the immune response of it or at least to furnish some data that could be used to facilitate developing of serologic test(s) later on. Antigens used were whole cell, stained whole cell and toxin. Samples of serum were taken from naturally or experimentally infected animals. The whole cell antigen was prepared by culturing the reference strain under conditions simulating those in-vivo. Hyperimmune serum was also prepared.
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