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Toxoplasmosis is a zoonotic infectious disease that can affect a variety of warm-blooded vertebrates, including humans and other warm-blooded domestic and wild animals. Antenatal statistics on this parasitic disease are thought to provide baseline data on the prevalence of T. gondii in pregnant women as well as for the planning and implementation of T. gondii diagnostic, control, and prevention programs. Therefore, this study was aimed to determine the seroprevalence of T. gondii infection and its risk factors among pregnant women attending Maternal, and child health (MCHs) on the emphasis of screening and management of pregnant women in Burao city, Somaliland.Objectives:To assess Toxoplasmosis seroprevalence in relation to knowledge and practice among pregnant women in Burao city, Somaliland, 2023. The overall positivity of against T. gondii in the study population was 67 (16%). Only 41.6% of the respondents have heard about the disease. Lack of knowledge significantly increasedthe risk of infection. This study improved knowledge of T. gondii infection exposure in Burao, Somaliland, as well as the relative significance of numerous risk variables.