The present study was planned to investigate the level of cholesterol in obese and non-obese individuals according to their BMI and age in different population residing in Karachi. A total of 40 subjects 18-55 years of age participated in the study, among which 30 subjects (Age=29.47±1.99) were obese (BMI=35.41±0.878) and 10 were control with (age =21.00±0.547) and BMI =19.96±0.432. An overnight fasting blood sample was obtained and serum total Cholesterol (T-CH) level was estimated. Obese persons having Mean T-CH=202.6±14.3 and control having Mean T-CH=173.3±14.0.The data was further divided into males and female. Obese females were 11 with their BMI 31.836± 0.21, mean age ranged at 28.73±4.04 and T-CH 191.8±21.8. While that of controls females were 6 in number with their BMI ranged at 19.183±0.507, age 21.000±0.894 and Cholesterol 148.7±16.0 in controls. Total number of obese males were 19 with their BMI ranged at 37.49± 1.14, age ranged at 29.89±2.19 and cholesterol level ranged at 208.89±19.1. Control males were 4 in number with their BMI ranged at 21.125±0.075, age 21.250±0 and their T-CH 128.7±8.72.