This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and…mehr
This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma.
Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.
Protocols for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion.- Collection of plasma samples in areas with limited healthcare access.- Blood Collection Processing & Handling for Plasma and Serum Proteomics.- Preparation of cryoprecipitate and cryo-depleted plasma for proteomic research analysis.- High throughput proteome profiling of plasma and native plasma complexes using native chromatography.- High-throughput and in-depth proteomic profiling of 5 µL plasma and serum using TMTpro 16plex.- In depth data-independent acquisition proteomics of human plasma identifies low abundant proteins.- In-depth Blood Proteome Profiling by Extensive Fractionation and multiplexed quantitative mass spectrometry.- Early cancer biomarker discovery using DIA-MS proteomic analysis of EVs from peripheral blood.- Strategies to enrich, identify and characterize glycoproteome in blood plasma using Liquid Chromatography High Resolution Mass Spectrometry.- Proteome analysis of whole blood collected by volumetric absorptive microsampling.- Proteomic applications and considerations: from research to patient care.- Immunoaffinity mass spectrometry diagnostic tests for multi-biomarker assays.- Secretome profile of leukocyte-platelet rich fibrin (L-PRF) membranes.- Quantification of proteins in blood by absorptive microtiter plate-based affinity purification coupled to liquid chromatography-mass spectrometry.- Glycomics-assisted Glycoproteomics Enables Deep and Unbiased N-Glycoproteome Profiling of Complex Biological Specimens. -The Small Protein Enrichment Assay (SPEA) for analysis of low abundance peptide hormones in plasma.- In-depth proteomic analysis of blood circulating small extracellular vesicles.- Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-based Proteomic Identification.- Analysis of ExtracellularVesicle and Contaminant Markers in Blood Derivatives Using Multiple Reaction Monitoring.- Generation of red blood cell nanovesicles as a delivery tool.- Progress in targeted mass spectrometry (parallel accumulation-serial fragmentation) and its application in plasma/serum proteomics.- Offline peptide fractionation and parallel reaction monitoring MS for the quantitation of low-abundance plasma proteins.- Profiling Serum Intact N-Glycopeptides Using Data-Independent Acquisition Mass Spectrometry.- Semi-automated lectin magnetic bead array (LeMBA) for translational serum glycoprotein biomarker discovery and validation.- Accessing antibody-reactivities in serum or plasma to (auto-)antigens using multiplexed bead-based protein immunoassays.- Absolute quantitative targeted proteomics assays for plasma proteins.- Rapid and quantitative enrichment of peptides from plasma for mass spectrometric analysis.- Comprehensive targeted lipidomic profilingfor research and clinical applications.- Multiplexed bead-based peptide immunoassays for the detection of antibody reactivities.- Array-based multiplex and high-throughput serology assays.- Bioinformatic tools and knowledgebases to assist generating targeted assays for plasma proteomics.- Use of Longitudinal Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early Detection.
Protocols for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion.- Collection of plasma samples in areas with limited healthcare access.- Blood Collection Processing & Handling for Plasma and Serum Proteomics.- Preparation of cryoprecipitate and cryo-depleted plasma for proteomic research analysis.- High throughput proteome profiling of plasma and native plasma complexes using native chromatography.- High-throughput and in-depth proteomic profiling of 5 µL plasma and serum using TMTpro 16plex.- In depth data-independent acquisition proteomics of human plasma identifies low abundant proteins.- In-depth Blood Proteome Profiling by Extensive Fractionation and multiplexed quantitative mass spectrometry.- Early cancer biomarker discovery using DIA-MS proteomic analysis of EVs from peripheral blood.- Strategies to enrich, identify and characterize glycoproteome in blood plasma using Liquid Chromatography High Resolution Mass Spectrometry.- Proteome analysis of whole blood collected by volumetric absorptive microsampling.- Proteomic applications and considerations: from research to patient care.- Immunoaffinity mass spectrometry diagnostic tests for multi-biomarker assays.- Secretome profile of leukocyte-platelet rich fibrin (L-PRF) membranes.- Quantification of proteins in blood by absorptive microtiter plate-based affinity purification coupled to liquid chromatography-mass spectrometry.- Glycomics-assisted Glycoproteomics Enables Deep and Unbiased N-Glycoproteome Profiling of Complex Biological Specimens. -The Small Protein Enrichment Assay (SPEA) for analysis of low abundance peptide hormones in plasma.- In-depth proteomic analysis of blood circulating small extracellular vesicles.- Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-based Proteomic Identification.- Analysis of ExtracellularVesicle and Contaminant Markers in Blood Derivatives Using Multiple Reaction Monitoring.- Generation of red blood cell nanovesicles as a delivery tool.- Progress in targeted mass spectrometry (parallel accumulation-serial fragmentation) and its application in plasma/serum proteomics.- Offline peptide fractionation and parallel reaction monitoring MS for the quantitation of low-abundance plasma proteins.- Profiling Serum Intact N-Glycopeptides Using Data-Independent Acquisition Mass Spectrometry.- Semi-automated lectin magnetic bead array (LeMBA) for translational serum glycoprotein biomarker discovery and validation.- Accessing antibody-reactivities in serum or plasma to (auto-)antigens using multiplexed bead-based protein immunoassays.- Absolute quantitative targeted proteomics assays for plasma proteins.- Rapid and quantitative enrichment of peptides from plasma for mass spectrometric analysis.- Comprehensive targeted lipidomic profilingfor research and clinical applications.- Multiplexed bead-based peptide immunoassays for the detection of antibody reactivities.- Array-based multiplex and high-throughput serology assays.- Bioinformatic tools and knowledgebases to assist generating targeted assays for plasma proteomics.- Use of Longitudinal Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early Detection.
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