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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. In lipidomics, the process of shotgun lipidomics (named by analogy with shotgun sequencing) uses analytical chemistry to investigate the biological function, significance, and sequelae of alterations in lipids and protein constituents mediating lipid metabolism, trafficking, or biological function in cells. Lipidomics has been greatly facilitated by recent advances in, and novel applications of, electrospray ionization mass spectrometry (ESI/MS). Lipidomics is a research field that studies the pathways and networks of cellular lipids in biological systems (i.e., lipidomes) on a large scale. It involves the identification and quantitation of the thousands of cellular lipid molecular species and their interactions with other lipids, proteins, and other moieties in vivo.