Silver nanoparticles have gained the spotlight for their application in diverse fields over the last decade. Their properties range from optical, conduction, electrochemical, and electromagnetic properties to biological like an antioxidant, antimicrobial, anti-inflammatory, anti-tumor, and so on. Many investigations on silver nanoparticles show that they have the potential for becoming a new trend in the biological field for their potential and effective antimicrobial and antibiofilm activity against a wide range of both gram-positive and gram-negative microbes. This study focused on the production of silver nanoparticles (AgNPs) via a biogenic or green synthesis approach using four different plant extracts, namely Catharanthus roseus, Clitoria ternatea Azadirachta indica and Musa balbisiana. Antimicrobial disk diffusion assay, biofilms assay, and MIC assay were performed for these AgNPs.