This volume looks at the latest technologies and methods--combined with new genetic tools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sections: Section one covers muscle stem cells and progenitor cells; Section Two discusses animal models for muscle stem cells and regeneration; and Section Three explores bioinformatics and imaging analysis for muscle stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary…mehr
This volume looks at the latest technologies and methods--combined with new genetic tools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sections: Section one covers muscle stem cells and progenitor cells; Section Two discusses animal models for muscle stem cells and regeneration; and Section Three explores bioinformatics and imaging analysis for muscle stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Skeletal Muscle Stem Cells and Regeneration: Methods and Protocols is a valuable tool for all researchers looking to expand their knowledge on skeletal muscle growth, repair, degeneration, aging, and regenerative medicine.
Flow Cytometer Analyses, Isolation, and Staining of Murine Muscle Satellite Cells.- Extra Eyelid-Derived Muscle Stem Cells.- Isolation, Culture, and Analysis of Zebrafish Myofibres and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis.- The Satellite Cell Colony Forming Cell Assay as a Tool to Measure Self-Renewal and Differentiation Potential.- Co-Cultures of Macrophages with Muscle Stem Cells or with Fibroadipogenic Precursor Cells from Regenerating Skeletal Muscle.- Measuring Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Muscle Stem Cells Using a Seahorse Analyzer: Applicability for Aging Studies.- High Throughput Screening of Mitochondrial Bioenergetics in Myoblasts and Differentiated Myotubes.- State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts.- Analyses of Mesenchymal Progenitors in Skeletal Muscle by Fluorescence-Activated Cell Sorting and Tissue Clearing.- In Vitro Maturation of Human Pluripotent Stem Cell-Derived Myotubes.- Differentiation of Human Fetal Muscle Stem Cells from Induced Pluripotent Stem Cells.- Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells.- Producing Engraftable Skeletal Myogenic Progenitors from Pluripotent Stem Cells via Teratoma Formation.- Techniques for Injury, Cell Transplantation, and Histological Analysis in Skeletal Muscle.- Murine Models of Tenotomy-Induced Mechanical Overloading and Tail-Suspension-Induced Mechanical Unloading.- Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique.- Skeletal Muscle Regeneration in Zebrafish.- Methods to Monitor Circadian Clock Function in Skeletal Muscle.- Visualizing MyoD Oscillations in Muscle Stem Cells.- In Vivo Modeling of Skeletal Muscle Diseases Using the CRISPR/Cas9 System in Rats.- In Vivo Investigation of Gene Function in Muscle Stem Cells by CRISPR/Cas9 Mediated Genome Editing.- Exons 45-55 Skipping Using Antisense Oligonucleotides in Immortalized Human DMD Muscle Cells.- In Vivo Evaluation of Exon 51 Skipping in hDMD/Dmd-null Mice.- Functional Analysis of MicroRNAs in Skeletal Muscle.- Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring (MRM) Profiling.- Single-Cell Transcriptomic Analysis of Mononuclear Cell Populations in Skeletal Muscle.- Assay for Transposase-Accessible Chromatin Using Sequencing (ATAC-seq) of Freshly Isolated Muscle Stem Cells.- Efficient Genome-Wide Chromatin Profiling by CUT&RUN with Low Numbers of Muscle Stem Cells.- Epitranscriptome Mapping of N6-Methyladenosine using m6A Immunoprecipitation with High Throughput Sequencing in Skeletal Muscle Stem Cells.- Visualization of RNA Transcripts in Muscle Stem Cells Using Single-Molecule Fluorescence In Situ Hybridization.- Tissue Clearing and Confocal Microscopic Imaging for Skeletal Muscle.- Three-Dimensional Imaging Analysis for Skeletal Muscle.
Flow Cytometer Analyses, Isolation, and Staining of Murine Muscle Satellite Cells.- Extra Eyelid-Derived Muscle Stem Cells.- Isolation, Culture, and Analysis of Zebrafish Myofibres and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis.- The Satellite Cell Colony Forming Cell Assay as a Tool to Measure Self-Renewal and Differentiation Potential.- Co-Cultures of Macrophages with Muscle Stem Cells or with Fibroadipogenic Precursor Cells from Regenerating Skeletal Muscle.- Measuring Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Muscle Stem Cells Using a Seahorse Analyzer: Applicability for Aging Studies.- High Throughput Screening of Mitochondrial Bioenergetics in Myoblasts and Differentiated Myotubes.- State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts.- Analyses of Mesenchymal Progenitors in Skeletal Muscle by Fluorescence-Activated Cell Sorting and Tissue Clearing.- In Vitro Maturation of Human Pluripotent Stem Cell-Derived Myotubes.- Differentiation of Human Fetal Muscle Stem Cells from Induced Pluripotent Stem Cells.- Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells.- Producing Engraftable Skeletal Myogenic Progenitors from Pluripotent Stem Cells via Teratoma Formation.- Techniques for Injury, Cell Transplantation, and Histological Analysis in Skeletal Muscle.- Murine Models of Tenotomy-Induced Mechanical Overloading and Tail-Suspension-Induced Mechanical Unloading.- Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique.- Skeletal Muscle Regeneration in Zebrafish.- Methods to Monitor Circadian Clock Function in Skeletal Muscle.- Visualizing MyoD Oscillations in Muscle Stem Cells.- In Vivo Modeling of Skeletal Muscle Diseases Using the CRISPR/Cas9 System in Rats.- In Vivo Investigation of Gene Function in Muscle Stem Cells by CRISPR/Cas9 Mediated Genome Editing.- Exons 45-55 Skipping Using Antisense Oligonucleotides in Immortalized Human DMD Muscle Cells.- In Vivo Evaluation of Exon 51 Skipping in hDMD/Dmd-null Mice.- Functional Analysis of MicroRNAs in Skeletal Muscle.- Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring (MRM) Profiling.- Single-Cell Transcriptomic Analysis of Mononuclear Cell Populations in Skeletal Muscle.- Assay for Transposase-Accessible Chromatin Using Sequencing (ATAC-seq) of Freshly Isolated Muscle Stem Cells.- Efficient Genome-Wide Chromatin Profiling by CUT&RUN with Low Numbers of Muscle Stem Cells.- Epitranscriptome Mapping of N6-Methyladenosine using m6A Immunoprecipitation with High Throughput Sequencing in Skeletal Muscle Stem Cells.- Visualization of RNA Transcripts in Muscle Stem Cells Using Single-Molecule Fluorescence In Situ Hybridization.- Tissue Clearing and Confocal Microscopic Imaging for Skeletal Muscle.- Three-Dimensional Imaging Analysis for Skeletal Muscle.
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