This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known…mehr
This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells.
In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging.- Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells.- Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction.- Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction.- Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells.- Isolation and Characterization of Cutaneous Epithelial Stem Cells.- Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin.- Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells.- Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis.- Isolation and Cultivation of Epidermal (Stem) Cells.- One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen.- Isolation and Cultivation of Skin-Derived Precursors.- Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells.- Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor.- Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair.- Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet.- Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering.- Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration.- Skin Wound Healing: Refractory Wounds and Novel Solutions.- Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice.- Melanoblasts as MultipotentCells in Murine Skin.- Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro.- Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells.- Isolating Immune Cells from Mouse Embryonic Skin.- Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells.- High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy.- High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications.- Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis.- Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells.- Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research.- Isolation of Cancer Stem Cells from Squamous Cell Carcinoma.- Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells.- Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells.
In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging.- Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells.- Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction.- Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction.- Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells.- Isolation and Characterization of Cutaneous Epithelial Stem Cells.- Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin.- Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells.- Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis.- Isolation and Cultivation of Epidermal (Stem) Cells.- One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen.- Isolation and Cultivation of Skin-Derived Precursors.- Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells.- Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor.- Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair.- Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet.- Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering.- Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration.- Skin Wound Healing: Refractory Wounds and Novel Solutions.- Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice.- Melanoblasts as MultipotentCells in Murine Skin.- Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro.- Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells.- Isolating Immune Cells from Mouse Embryonic Skin.- Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells.- High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy.- High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications.- Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis.- Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells.- Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research.- Isolation of Cancer Stem Cells from Squamous Cell Carcinoma.- Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells.- Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells.
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