Buffalo and cattle are mostly reared for milk production the mastitis decreases the milk production and sometimes causes bloody milk alone or mixed with mucus may cause milk unfit for consumption. It of lactation and unwanted culling of dairy buffaloes one of the most important reasons for termination. Mastitis namely, clinical and subclinical, is an economically damaging disease of the dairy industry, which causes physical, chemical and bacteriological alteration in the milk and blood along with morphopathological changes in the mammary gland. The causes of mastitis involve a complex relationship of three major factors, that are: host resistance, bacterial agents and the environmental factors. Throughout the book emphasis is placed on through light on mastitis with the macroscopic and microscopic recognition of all diagnostic methods of mastitis. where appropriate, the chemotherapy, control and prevention of mastitis .Specific chapter of practical research for bacteriological and biochemical study on subclinical mastitis on buffaloes. These books discuss new strategies to diagnosis of sub- clinical mastitis in buffalo. in the end specific chapter for recommendations and conclusio