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A cross-sectional entomological study on species composition and significance of Insecticide Treated Mosquito Nets (ITNs) used for malaria vector control were conducted in four randomly selected localities of, Meskan Woreda, Guragie Zone, Southern Ethiopia. Larval and adult collections were carried out, from different sites throughout the study period to identify species composition. In order to get adequate protection, governmental sectors and other concerned organizations; center of attention would be the creation of awareness on utilization, supply and evaluation of the longevity of insecticide on the net as well as durability of the net at operational level.