SPECTROPHOTOMETRY IN PHARMACEUTICAL ANALYSIS - Ahmed, Shabbir;Rasul, Akhtar;Masood, Zeeshan
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A simple spectrophotometric method for determination of cefaclor in raw and dosage forms based on reaction with ninhydrin is developed, optimized and validated. The purple color (Ruhemenn s purple) resulted from the reaction was stabilized and measured at 560 nm.The method optimization was essential as many procedural parameters influenced the accuracy of determination all variables including the reagent concentration, heating time, reaction temperature, color stability period, and cefaclor/ninhydrin ratio were studied in order to optimize the reaction conditions. No interference was observed…mehr

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Produktbeschreibung
A simple spectrophotometric method for determination of cefaclor in raw and dosage forms based on reaction with ninhydrin is developed, optimized and validated. The purple color (Ruhemenn s purple) resulted from the reaction was stabilized and measured at 560 nm.The method optimization was essential as many procedural parameters influenced the accuracy of determination all variables including the reagent concentration, heating time, reaction temperature, color stability period, and cefaclor/ninhydrin ratio were studied in order to optimize the reaction conditions. No interference was observed from common pharmaceutical adjuvant. The developed method is easy to use, accurate and highly cost-effective for routine studies relative to HPLC and other techniques.
Autorenporträt
El análisis de drogas juega un papel importante en el desarrollo de las drogas, su fabricación y su uso terapéutico. Las industrias farmacéuticas se basan en el análisis químico cuantitativo para asegurarse de que la materia prima utilizada y los productos finales obtenidos cumplen las especificaciones requeridas.