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One of the most commonly used dyes, Rhodamine B (RhB) is widely used in industrial purposes, such as printing, dyeing in textile, paper, paints, etc. It is a synthetic and basic dye.It is used as a staining fluorescent dye. RhB is bright-red in color. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy.