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Mikania scandens(L.)Willd. is a rapidly growing herbaceous hemp vine belongs to the family Asteraceae. Present study is concentrated on anthelmintic, antimicrobial and phytochemical aspects on Mikania scandens(L.)Willd. The phytochemicals of leaves of Mikania scandens(L.)Willd. was extracted using polar and non polar solvents viz, Hexane and Methanol. The antimicrobial activity of extracts were tested against Gram- positive and Gram- negative bacteria and two fungal strain. The essential oil also screened against test strains and show better antimicrobial activity. In vitro experiment was performed to evaluate anthelmintic activity of extracts, using Pheretima posthuma. The polar solvent shows better activity than the standard Albendazole. The methanol extracts of Mikania scandens(L.)Willd. nine compounds were analyzed by HPLC. The essential oil is obtained by hydrodistillation of the whole plant of Mikania scandens , fifteen compounds were analyzed by GC-MS. Various kinds of monoterpenoids, diterpenoids and sesquiterpenoids were the major phytochemicals detected.