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The present investigation is envisaged to study the production of alkaline and thermostable polygalacturonase using isolated soil Bacillus subtilis(C4) by submerged fermentation. A total of 66 bacterial colonies were isolated from six sampling sites. Out of 66 isolates 8 isolates showed polygalacturonase activity and C4 isolate showed maximum activity. The new isolate polygalacturonase potential was tested against three polygalacturonase producing microorganism. Comparatively isolate C4 showed maximum enzyme activity hence it was chosen for in depth study. The isolate C4 was characterized and identified as Bacillus subtilis C4. The new Bacillus subtilis was used for polygalacturonase production under submerged ferementation Growth profile of the above isolate was studied. The results revealed that the organism was showing maximum log phase till 24hr period after which the growth gradually reduced and remained stable till 72h indicating stationary phase at 400C and pH 11.0. Process parameters like incubation time (19.65U/mL), temperature (28.08 U/mL),pH 7.0, inoculum volume(1mL), inoculum age (24h) and agitation (150rpm) were studied for maximum production of polygacturonase.