Bacillus sphaericus in this work is highly promising strain to produce highly active mosquitocide in the same fermentation proccess with highly tolerant alkaline protease wich enhance the toxicity against mosquito larvae at the same time used economically in alot of industrial applications; pesticides, detergents, silver recovery, tanning and many other important applications which depends on highly alkaline pH. This approach safe invironmentally rather than the dangerous chemical pesticides and chemical industrial reagent, take tour with this work and use safe and economic both pesticide and alkaline protease.