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The naturally occurring xanthone glycoside mangiferin has been isolated by column chromatography from the ethanolic extract of stem bark of Mangifera indica. Mangiferin was further converted to Mangiferin-octa-Acetate, Mangiferin-tetra-benzoate, 5-(N-phenylamino methyleno) mangiferin, 5-(N-p-chlorophenylamino methyleno) mangiferin, 5-(N-2-methyl phenylamino methyleno) mangiferin, 5-(N-p-Methoxy phenylamino methyleno) mangiferin, 5-(N, N-Diphenylamino methyleno) mangiferin, 5-(N- -Napthylamino methyleno)-mangiferin, 5-(N-4-methyl phenylamino methyleno) mangiferin, 5-(N-m-Nitrophenylamino methyleno) mangiferin, 5-(N-p-flurophenylamino methyleno) mangiferin, Octa-o-methyl mangiferin, Mangiferin-9- Oxime. Mangiferin and its analogues were characterized by mp, Rf , UV, IR, and NMR spectral analyses. These compounds were screened for antimicrobial activity. Among the compound tested, only MPAMM-4, a mangiferin derivative was found to be more effective than other compounds against both bacteria (Escherichia coli) and fungus (Aspergillus niger).